13 games
| Date | Site | Opp AP | Opponent | UL AP | W/L | UL | Opp | OT | Overall | Conf | Attendance | City | State | Stadium | Event |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 08/30/2025 | H | Eastern Kentucky | W | 51 | 17 | 1-0 | 50,536 | Louisville | Ky. | L&N Federal Credit Union Stadium | |||||
| 09/05/2025 | H | James Madison | W | 28 | 14 | 2-0 | 48,717 | Louisville | Ky. | L&N Federal Credit Union Stadium | |||||
| 09/20/2025 | H | Bowling Green | W | 40 | 17 | 3-0 | 49,482 | Louisville | Ky. | L&N Federal Credit Union Stadium | |||||
| 09/27/2025 | A | Pittsburgh | W | 34 | 27 | 4-0 | 1-0 | 45,301 | Pittsburgh | Pa. | Acrisure Stadium | ||||
| 10/04/2025 | H | 24 | Virginia | L | 27 | 30 | OT | 4-1 | 1-1 | 50,032 | Louisville | Ky. | L&N Federal Credit Union Stadium | ||
| 10/17/2025 | A | 2 | Miami, Fla. | W | 24 | 21 | 5-1 | 2-1 | 66,573 | Miami | Fla. | Hard Rock Stadium | |||
| 10/25/2025 | H | Boston College | 19 | W | 38 | 24 | 6-1 | 3-1 | 50,320 | Louisville | Ky. | L&N Federal Credit Union Stadium | |||
| 11/01/2025 | A | Virginia Tech | 16 | W | 28 | 16 | 7-1 | 4-1 | 54,030 | Blacksburg | Va. | Lane Stadium | |||
| 11/08/2025 | H | California | 14 | L | 26 | 29 | OT | 7-2 | 4-2 | 51,381 | Louisville | Ky. | L&N Federal Credit Union Stadium | ||
| 11/14/2025 | H | Clemson | 19 | L | 19 | 20 | 7-3 | 4-3 | 51,234 | Louisville | Ky. | L&N Federal Credit Union Stadium | |||
| 11/22/2025 | A | Southern Methodist | L | 6 | 38 | 7-4 | 4-4 | 32,713 | Dallas | Texas | Gerald J. Ford Stadium | ||||
| 11/29/2025 | H | Kentucky | W | 41 | 0 | 8-4 | 4-4 | 50,634 | Louisville | Ky. | L&N Federal Credit Union Stadium | ||||
| 12/23/2025 | N | Toledo | W | 27 | 22 | 9-4 | 4-4 | 15,329 | Boca Raton | Fla. | Flagler Credit Union Stadium | Boca Raton Bowl of Beans |